| Group | Club/Team Name | Team ID | Head Coach |
| Seed 1 | Tormenta FC Academy - 01 TFC 09 Girls |
03035-001CG3U-0084 | Miguel Lopez |
| Seed 4 | Cainhoy Athletic Soccer Club CASC 08 Fury |
03006-001CG3U-2207 | Dominique Lopez |
| Seed 5 | Bulls Soccer Club KEEP U13 Bulls Girls Red 2008 |
02012-0031G3U-2486 | Stephen Roach |
| Seed 8 | South Carolina United FC - Competitive SCUFC 08 Girls Challenge |
02208-2081G3U-0351 | Christine Martin |
| Seed 2 | CESA Competitive CESA 08G Challenge |
01001-0011G3U-0016 | Waleed Berber |
| Seed 3 | Charlotte Independence Soccer Club CISC South 09 girls PE |
02014-0011G3U-0584 | James Lockhart |
| Seed 6 | South Carolina Surf Soccer Club SC Surf 09 Elite |
03008-0011G3U-0213 | Aaron McGarvey |
| Seed 7 | Florence SA - 01 Florence 08 Girls |
02001-0011G3U-1276 | Thomas Fluharty |